RECOMBINANT DNA LABORATORY |
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The Recombinant DNA laboratory
as a Core Facility of the Sealy Center for Molecular Science offers
a variety of technical services designed to benefit and enhance
research programs at the University of Texas Medical Branch. Recombinant
DNA technology has evolved from a specialty into a integral part
of the research strategy for programs that transcend the various
disciplines within both basic and clinical research. The increased
emphasis on the use of molecular approaches to address the specific
aims of today's research programs has required a level of sophistication
in experimental design that only recently would have been considered
avant-garde. The role of the Recombinant DNA Laboratory is to provide technical support that will facilitate the successful attainment of these research aims by providing state-of-the-art reagents and technical support in the areas of recombinant DNA technology. Available services include plasmid DNA production, phage DNA purification (lambda and M13), competent cell preparation, transformation and screening, isolation of eukaryotic RNA and DNA, DNA sequencing, genomic and cDNA library construction , polymerase chain reaction technologies, vector/plasmid construction, bacterial expression and various types of mutagenesis (deletion, replacement or site-directed). The costs for specific services for UTMB faculty are listed
below.
FORMS
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| You must use the PRINT button on the form in order to process correctly. |
| Request for Service-General |
| Competent Cells See a list of available cells |
| Request for Service-Transformation |
| Request for Service-Plasmid Purification |
| Request for DNA-RNA Isolation |
| M13 RF DNA Purification | $92 | DNA Sequence Analysis (per reaction) | $10 |
| M13 Phage DNA Purification | $93 | Southern Blot Hybridization | $92 |
| Lambda Phage DNA Purification | $53 | Plasmid Purification | $92 |
| Eukaryotic DNA Isolation | $5 | PCR Reaction and Gel Analysis | $12 |
| Transformation and Screening | $17 | TA-Cloning |
$36 |
| Eukaryotic Total RNA Isolation | $16 | Site-Directed Mutagenesis | $131 |
| Competent Cell (0.5 ml) | $4 |